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Sreerama Kumar, P.
- Resistance and Susceptibility Pattern of Chickpea (Cicer arietinum L) Endophytic Bacteria to Antibiotics
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Authors
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1 Biological Control (ICAR), H. A. Farm Post, Hebbal, Bellary Road, Bangalore, 560 024, Karnataka, IN
2 Department of Agricultural Microbiology, University of Agricultural Sciences, Bangalore, Karnataka, IN
1 Biological Control (ICAR), H. A. Farm Post, Hebbal, Bellary Road, Bangalore, 560 024, Karnataka, IN
2 Department of Agricultural Microbiology, University of Agricultural Sciences, Bangalore, Karnataka, IN
Source
Journal of Biological Control, Vol 22, No 2 (2008), Pagination: 393-403Abstract
Five chickpea (Cicer arietinum) endophytic bacteria, identified as Erwinia herbicola, Enterobacter agglomeraus. Bacillus megaterium and Bacillus sp. and Bacillus circulans were tested for intrinsic antibiotic resistance in order to see if endophytes showed variation in resistance to antibiotics. The resistance pattern was compared with two rhizospheric bacteria viz. Psendomonas fluoresceus and B. subtilis in order to see if the susceptibility of endophytes differed with that of bacteria isolated from rhizosphere. The endophytes seemed to be less resistant to antibiotics. B. circulaus was susceptible to all antibiotics tested except amoxycillin (10μg). However B. megaterium and Bacillus sp, and E. agglomeraus showed some resistance, P. fluorescens and B. subtilis showed resistance to a wide range of antibiotics indicating that they could be better competitors in the rhizosphere. Preliminary screening was done to monitor B. megaterium and Bacillus sp. by using the observed antibiotic resistance. Out of the 25 ischolar_main/stem/leaf tissues tested, 10 tested positive for the presence of B. megaterium and 11 for Bacillus sp. However, they coutd not be reisolated from the stem tissue, 3 and 2 of the leaf samples showed presence of B. megaterium and Bacillus sp., respectively.Keywords
Antibiotics, Endophytic Bacteria, Resistance Pattern.- New, Safer Molecules and Biocontrol Technologies for Integrated Pest Management in Crops
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PDF Views:95
Authors
Affiliations
1 ICAR-National Bureau of Agricultural Insect Resources, Bengaluru 560 024, IN
1 ICAR-National Bureau of Agricultural Insect Resources, Bengaluru 560 024, IN
Source
Current Science, Vol 109, No 10 (2015), Pagination: 1766-1767Abstract
No Abstract.- Potential Use of Polyethylene Glycol in the Mass Production of Nonsynnematous and Synnematous Strains of Hirsutella thompsonii Fisher in Submerged Culture
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Authors
Affiliations
1 Project Directorate of Biological Control (ICAR), P. B. No. 2491, H. A. Fann Post, Bellary Road, Hebbal, Bangalore 560024, Karnataka, IN
1 Project Directorate of Biological Control (ICAR), P. B. No. 2491, H. A. Fann Post, Bellary Road, Hebbal, Bangalore 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 19, No 2 (2005), Pagination: 105-113Abstract
Polyethylene glycol (PEG 6000) at six different concentrations (1, 2, 3, 4, 5 and 6% w/v) in Sabouraud dextrose broth influenced both quality and quantity of the biomass of Hirsutella thompsonti (nonsynnematous) and H. thompsonti var. synnematosa (synnematous) in submerged culture. Whereas the number of colony-forming units (CFU), wet and dry weights of both nonsynnematous and synnematous strains increased, pellet number and size decreased with the increasing concentration of PEG in the medium. However, there was no significant effect of the chemical on conidial germination, although conidiation of biomass of both strains was slightly delayed. The intended aim of getting more of hyphal fragments and less of mycelial pellets with the chemical was achieved as reflected in the maximum CFU obtained with 6 per cent PEG in H. thompsonii (360.53 × 103/ml) and H. thompsonti var. synnematosa (237 × 103/ml). Compared with the respective controls, the highest level of PEG produced 6 and 2.8 times less number of fully-formed pellets in the nonsynnematous and synnematous strains, respectively.Keywords
Biomass, Fungal Pathogen, Hirsutella thompsonii, H. thompsonii var. Synnematosa, Mass Production, Pellets, Polyethylene Glycol, Submerged Culture.- Investigations on Sporothrix fungorum De Hoog&de Vries, a Newly Recorded Pathogen of Aceria guerreronis Keifer, the Coconut Eriophyid Mite
Abstract Views :226 |
PDF Views:110
Authors
Affiliations
1 Project Directorate of Biological Control (ICAR), P. B. No. 2491, H.A. Farm Post, Hebbal, Bellary Road, Bangalore 560 024, Karnataka, IN
1 Project Directorate of Biological Control (ICAR), P. B. No. 2491, H.A. Farm Post, Hebbal, Bellary Road, Bangalore 560 024, Karnataka, IN
Source
Journal of Biological Control, Vol 18, No 1 (2004), Pagination: 13-20Abstract
The hyphomycetous fungus, Sporothrix fungorum de Hoog&de Vries was discovered affecting the coconut eriophyid mite, Aceria gllerreronis Keifer in Karnataka and Tamil Nadu in India during 1999-2000 in up to 15 per cent of coconut samples. At 2xlOs, 2xl0· and 2xl07 conidia per ml the fungus could bring' about 47.03, 92.75 and 98.29 per cent mortalities, respectively, 96h after inoculation. It was able to grow and conidiate profusely on potato dextrose agar (PDA) as well as Sabouraud dextrose agar (SDA). The diameter of the colony increased significantly between 10 and 21 days of inoculation in both the media, reaching up to 35.83 and 36.00 mm on PDA and SDA, respectively. Among three different liquid media, viz., potato dextrose broth (PDB), Sabouraud dextrose broth (SDB) and an enrichment medium (EM), EM was the best in terms of wet and dry weights (per 100 ml) as well as conidia production in both stationary (6450.3 mg, 1602.3 mg and 71.7 x 106 / ml) and shake cultures (13416.7 mg, 3402.7 mg and 83.0 x 107 / mI). The present report gives new details about S. fungorum as well as its association with the coconut mite.Keywords
Aceria gllerrerollis, Biological Control, Coconut, Eriophyid Mite, Sporothrix fungorum.- Occurrence of Fusarium Coccophilum (Desm.) Wollenw.& Reinking on Sugarcane Whitefly, Aleurolobus barodensis (maskell) (homoptera: Aleyrodidae)
Abstract Views :214 |
PDF Views:145
Authors
Affiliations
1 Project Directorate of Biological Control (lCAR) P. B. No. 2491, H. A. Farm Post, Bellary Road, Hebbal Bangalore 560024, Karnataka, IN
1 Project Directorate of Biological Control (lCAR) P. B. No. 2491, H. A. Farm Post, Bellary Road, Hebbal Bangalore 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 14, No 1 (2000), Pagination: 49-50Abstract
Fusarium coccopllilum (Desm.) Wollenw.&Reinking was recorded as a fungal pathogen of the sugarcane whitefly, Aleuroiobus barodensis (Maskell) from Dhule and Pune areas of Maharashtra. The infection level during rainy season varied from 2.2 to 12.8 per cent. Its pathogenicity both to nymphs and pupae was confirmed. This is the first report of F. coccophiluln on sugarcane whitefly.Keywords
Aleuroiobus barodellsis, Fungal Pathogen. Fusarium Coccopllilum. Sugarcane Whilefly- Nematicidal Effect of Fungal Filtrates against Root-Knot Nematodes
Abstract Views :215 |
PDF Views:132
Authors
Affiliations
1 Project Directorate of Biological Control, Post Bag No. 2491, H. A. Farm Post, Bangalore 560024, Karnataka, IN
1 Project Directorate of Biological Control, Post Bag No. 2491, H. A. Farm Post, Bangalore 560024, Karnataka, IN